Scientist/Sr Scientist – Protein Production and Purification
On-sitePalo Alto, California, United States
Job Summary
Design, execute, and optimize recombinant protein expression workflows for antibodies, therapeutic proteins, and research reagents using transient mammalian and bacterial systems. Develop and execute purification processes for diverse proteins and conjugates via affinity, ion exchange, and size exclusion chromatography. Perform biochemical and biophysical characterization using SDS-PAGE, SEC, DLS, mass photometry, and isoelectric focusing to evaluate quality and stability. Maintain, optimize, and troubleshoot mammalian cell culture and production processes to ensure robust yields. Plan, prioritize, and coordinate protein production activities across multiple research programs while independently managing laboratory operations, equipment, and reagent inventories. Analyze experimental data, prepare technical reports, and present findings to cross-functional teams. Maintain comprehensive laboratory notebooks and inventory databases to ensure data integrity and regulatory compliance.
Required Qualifications
- Ph.D. in Biochemistry, Cell Biology, Molecular Biology, Protein Science, or a related discipline with 0-5 years of relevant postdoctoral and/or industry experience
- M.S. or B.S. in related discipline with 5+ years of relevant industry experience
- Demonstrated expertise in recombinant protein expression using transient mammalian expression systems (e.g., HEK293, CHO)
- Extensive hands-on experience in the purification of antibodies and diverse recombinant proteins using affinity, ion exchange, size exclusion, and other chromatographic techniques in both column- and batch-based formats
- Proficiency in operating, optimizing and troubleshooting protein purification platforms, including ÄKTA FPLC systems
- Strong foundation in protein biochemistry
- Hands-on experience with biochemical and biophysical characterization techniques, including SDS-PAGE, SEC, DLS, UV-Vis spectroscopy, mass photometry, isoelectric focusing (IEF), and related analytical methods
- Demonstrated ability to independently design, execute, optimize, troubleshoot, and interpret recombinant protein expression, purification, and characterization experiments
- Proven ability to optimize protein production workflows and implement innovative approaches to improve productivity, protein quality, and throughput
- Excellent organization skills
- Meticulous attention to detail
- Commitment to maintaining accurate experimental records and documentation
- Strong written and verbal communication skills
- Ability to effectively collaborate and communicate scientific findings across multidisciplinary research teams
Desired Qualifications
- Experience with bacterial expression systems
- Experience with KingFisher or other automated purification platforms
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