Research Assistant IV
$56,846–$72,488 year
On-siteBurnet, Texas, United States
Job Summary
Conduct independent and directed scientific studies supporting a Principal Investigator's research on immune cell isolation and multiome analysis. Design and perform experiments, including lentiviral transduction and flow cytometry, while developing and standardizing new molecular protocols for DNA, RNA, and protein extractions. Analyze data through statistical and graphical methods, interpret flow cytometry results, and prepare libraries for ATAC-seq and ChIP-seq sequencing. Maintain accurate laboratory records, ensure quality and reproducibility checks, and manage inventory of supplies and equipment. Assist in training postdoctoral fellows and junior personnel, and contribute to reports, publications, and laboratory meetings.
Required Qualifications
- Bachelor's degree or higher in chemistry, biology, molecular biology, biotechnology, or biochemistry
- 1 year of experience in conducting independent and directed scientific studies and research in a lab setting
- 1 year of experience in isolating and stimulating immune cells in a biosafety level 2 laboratory and optimizing subsequent molecular protocols to be used to assess these cells
- 1 year of experience in working independently and as part of a team in an academic research lab
- 1 year of experience in isolating inflamed blood cells from small amounts (less than 3 mL) of peripheral blood
- 1 year of experience in isolating RNA, DNA, chromatin, nuclear lysates, and cytoplasmic lysates from cells with subsequent quality assessment of isolated materials
- 1 year of experience in performing Western blot analysis with proficiency in Empiria Studio
- 1 year of experience in performing flow cytometry to measure expression of proteins on a single cell level using antibody staining for extracellular and intracellular epitopes with proficiency in DB FACSDiva and FlowJo for quantification and analysis
- 1 year of experience in development of research protocols
- 1 year of experience in optimizing biochemical protocols with primary cells derived from inflamed tissues
- 2 years of experience in providing Material Safety Data Sheet (MSDS) training
- 2 years of experience in handling disposal of chemicals
- 1 year of experience collaborating and coordinating with a physician around experimental logistics of obtaining research samples from a clinical encounter
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