PhD/Researcher (m/f/d) Computational Fluid Dynamics & Experimental Validation

PhD student will establish and characterize engineered murine leukemia models from preB cells and hematopoietic stem and progenitor cells (HSPCs) using retroviral gene transfer and CRISPR/Cas9 gene editing, and validate models with molecular read-outs and flow cytometry. You will support functional genomics workflows, including sample preparation and single-cell library preparation for RoCK&ROI-Seq (BD Rhapsody-based workflows), and conduct drug response profiling in high-throughput formats. You will perform in vitro and in vivo assays to study lineage plasticity and therapy escape, including differentiation/co-culture experiments and mouse transplantation/immunotherapy experiments (CD19xCD3 BiTE and CAR-T cells) in collaboration with partners of the CATCH ALL research unit. Ideal candidates hold a MSc or equivalent, have hands-on experience with cell culture and molecular biology methods (PCR, cloning, transfection), and familiarity with flow cytometry and genetic engineering (viral transfer/CRISPR); experience with NGS library prep is advantageous. The position offers access to state-of-the-art infrastructure in Kiel, with training opportunities in genomic engineering, murine models, systems immunology and integrative bioinformatics, under the German E13 TV-L scale (65%) contingent on collective bargaining terms.