Molecular Biology Scientist/Senior Associate Scientist
$93,463–$122,670 year
On-sitePhiladelphia, Pennsylvania, United States
Job Summary
Execute viral vector plasmid and helper plasmid design, construction, and routine molecular cloning workflows to support gene overexpression, knock-down, and knock-out studies. Perform mammalian cell culture, transient transfection under BSL-2 conditions, and generate engineered cell lines for vector production and single-cell cloning. Maintain accurate plasmid records, sequence files, and electronic lab notebooks while collaborating on small-scale vector production and functional evaluations using qPCR, ELISA, Western blot, and flow cytometry. Independently plan daily execution, analyze data, and propose next steps to project leads. Join the Vector Biology team in Philadelphia to advance lentiviral vector design and cell therapy programs.
Required Qualifications
- M.S. with 5+ years or Ph.D. with 0-2 years in a related field
- hands-on molecular cloning, cell engineering and mammalian cell culture
- Hands-on experience in molecular biology techniques (cloning, plasmid prep, sequence analysis and construct verification)
- Experience with mammalian cell culture and transient transfection
- familiarity with BSL-2 practices and documentation
- Experience generating engineered cell lines (stable expression and/or CRISPR KO/KI) including cloning and basic screening
- Exposure to viral vector production and/or viral vector workflows (LVV)
- Experience with titering and functional readouts (e.g., transduction efficiency and gene expression)
- Experience with qPCR/dPCR, flow cytometry, Western blot, and ELISA for tittering and functional assays
- Strong ELN documentation, sample tracking, and ability to follow protocols/SOPs with attention to detail
- Adaptable to a fast-paced environment
- effective prioritization, collaboration, and communication
- CRISPR/Cas gene editing for KO/KI or multiplexed cell lines
- Familiarity with viral vector process development concepts (upstream and/or downstream)
- Exposure to NGS or Nanopore sequencing for vector or genome characterization
- Organized and detail-oriented
- able to manage multiple experiments/projects
- Analytical and problem-solving skills to troubleshoot and implement improvement
- Collaborative; able to work effectively within a team environment
- Maintain accurate ELN, sample tracking, and inventory records
- Clear written and verbal communication for documentation and data presentation
- based in Philadelphia, PA
Desired Qualifications
- Scientist preferred
- Exposure to viral vector production and/or viral vector workflows (LVV preferred)
- Tech transfer and/or CMC documentation experience
- Clear written and verbal communication for documentation and data presentation
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